Human EphA4 / HEK8 Protein (aa 570-986, His & GST Tag) 11314-H20B1 from Sino Biological, Inc.

Product Specs
ItemHuman EphA4 / HEK8 Protein (aa 570-986, His & GST Tag)
CompanySino Biological, Inc.
Price Supplier Page View Company Product Page
Catalog Number11314-H20B1
Conjugate/TagN-GST, His
Purity> 90 % as determined by SDS-PAGE
TypeRecombinant protein
Molecular WeightThe recombinant human EPHA4 /GST chimera consists of 654 amino acids and has a calculated molecular mass of 75 kDa. The recombinant protein migrates as an approximately 67 kDa band in SDS-PAGE under reducing conditions
SpeciesHuman
Molecule NameEphA4
Source/Expression SystemBaculovirus-Insect Cells
NCBI Full Gene NameEPH receptor A4
NCBI Gene AliasesEK8, HEK8, SEK, TYRO1
Activity1. The specific activity was determined to be 17 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized human EPHA4 (aa 570-986) (Cat: 11314-H20B1) at 10 µg/ml (100 µl/well) can bind biotinylated human EphrinA5-His with a linear range of 0.625-5.0 µg/ml
FormatSupplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol, 3mM DTT

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(1) Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4Cell chemical biologyJune 16, 2022Wolff DW, Deng Z, Bianchi-Smiraglia A, Foley CE, Han Z, Wang X, Shen S, Rosenberg MM, Moparthy S, Yun DH, et al.mM ATP and appropriate amount (0.05-0.1 μg) of purified EPHA4 recombinant protein (Sino Biological, #11314-H20B1). 1 μg purified GST-GMPR protein was then added into the kinase cocktail for 1 hour incubation at room

Human EphA4 / HEK8 Protein (aa 570-986, His & GST Tag)